biotinylated goat anti osteopontin antibody  (R&D Systems)

Journal: Bone

Article Title: Modeling-based bone formation transforms trabeculae to cortical bone in the sclerotic areas in Buschke-Ollendorff syndrome. A case study of two females with LEMD3 variants.

doi: 10.1016/j.bone.2020.115313

Figure Lengend Snippet: Fig. 4. Histological examination of the transiliac bone biopsy obtained from subject 1. The illustrated adjacent sections are either Masson-Goldner trichrome stained (A–B, G, I) or immunostained for osteopontin (C–F, H). A: Overview image of the complete transiliac bone biopsy. Frames mark the two sclerotic areas investigated in detail. B-C: Illustrated the sclerotic area in the bone marrow cavity. Note the presence of pores within the sclerotic area. D: Illustrates the presence of MBF BSU with smooth osteopontin-rich cement lines (yellow arrowheads) at the edge of the sclerotic bone and osteons (#) within the sclerotic bone. High magnifications of the framed area are shown in F–G. Here the scalloped osteopontin-poor cement lines of RBF BSU are visible (green arrowheads). E: Illustrated smooth osteopontin-rich cement lines embedded in the sclerotic bone, as well as osteons. Note that these smooth osteopontin-rich cement lines represent oRBF BSU (blue arrowheads), as they are extended scalloped osteopontin-poor cement lines. High magnifications of the framed area are shown in H-I. Here the scalloped osteopontin-pore cement lines of remodeling-based BSU are more visible. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The sections were then incubated with biotinylated goat anti-osteopontin antibodies (BAF1433, R&D systems, Minneapolis, MN, US) diluted in Renoir Red (PD904, Biocare Medical, Concord, CA, US), which were detected with alkaline phosphatase conjugated streptavidin (016-050-084, Jackson ImmunoResearch, Suffolk, UK) and visualized with Liquid Permanent Red (DAKO, Glostrup, DK).

Techniques: Staining

Journal: Bone

Article Title: Modeling-based bone formation transforms trabeculae to cortical bone in the sclerotic areas in Buschke-Ollendorff syndrome. A case study of two females with LEMD3 variants.

doi: 10.1016/j.bone.2020.115313

Figure Lengend Snippet: Fig. 5. Histological examination of the sclerotic bone thickening the cortical bone within the transiliac bone biopsy obtained from subject 1. The illustrated adjacent sections are either Masson-Goldner trichrome stained (A, C, E, G) or immunostained for osteopontin (B, D, F, H). A–B: Overview of the thickened cortex and the sclerotic bone on its endosteal surface. High magnifications of the frames area are shown in C–H. C–D: Endocortical sclerotic bone with MBF BSU with smooth osteopontin-rich cement lines (yellow arrowheads) at the edge of the sclerotic bone and osteons (#) within the sclerotic bone. High magnifications of the framed area are shown in E–F. Here the scalloped osteopontin-poor cement lines of hemi-osteonal and osteonal RBF BSU are visible (green arrowheads). G–H: Intracortical osteons with scalloped osteopontin-poor cement lines. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The sections were then incubated with biotinylated goat anti-osteopontin antibodies (BAF1433, R&D systems, Minneapolis, MN, US) diluted in Renoir Red (PD904, Biocare Medical, Concord, CA, US), which were detected with alkaline phosphatase conjugated streptavidin (016-050-084, Jackson ImmunoResearch, Suffolk, UK) and visualized with Liquid Permanent Red (DAKO, Glostrup, DK).

Techniques: Staining